Introduction

High Throughput Phenomics platform (HTP) enables, High Content Screening (HCS), that combines automated imaging and quantitative data analysis in a high-throughput format suitable for large-scale applications such as drug discovery and systems biology utilising high throughput image phenotyping.

HTP support medium-scale high throughput/content screening campaigns. HTP hold diverse targeted small molecule probes/libraries and genome wide siRNA/shRNA libraries in 384-well Ready-To-Screen format. Overall, HTP can provide novel systems-level insight for your complex biological questions.

Key Expertise

Cell Painting – Detailed Morphometric Characterisation based on changes in sub-cellular components

Osteosarcoma cells were treated with small molecules and at the end of 48-hours, cells were stained for 8 sub-cellular organelles using 6 specific dyes.

Co-culture Modeling – Compounds Targeting Specific Sub-populations of Head & Neck Tumour

DMSO Pri. (Green) and Met (Red) cells	Compound 1 targets Pri. (Green) cells	Compound 2 targets Met (Red) cells	Compound 3 targets both Pri. and Met

Primary and Metastatic Head & Neck tumour cells were co-cultured. Compound 1 selectively kills primary cells, compound 2 selectively kills metastatic cells, and compound 3 kills both primary and metastatic cells.

Marker Expression – Compounds Inhibiting β-catenin in Colorectal Tumour

High Content Screening – Nucleus (Blue) + β-catenin (Green) + Cell Marker (Red)

DMSO Control	Compound 1	Compound 2

Protein Activation – Compounds Dephosphorylating ERK in Tumour

High Content Screening – Nucleus (Blue) + pERK (Green)

Uninduced Control	Induced Control	Induced + Compound 1	Induced + Compound 2

Cellular Activity – Compounds affecting Neurite Outgrowth in Amyotrophic Lateral Sclerosis Change Negative Control to Compound 1, Positive Control to Compound 2

DMSO Control	Compound 1	Compound 2

3D Tumour Spheroid Modelling – Compounds Targeting Colorectal Cancer

	Compound 1 Compound 2
Brightfield	3D-live cell detection-Calcein AM staining
Brightfield	3D-live/dead cell detection-Calcein AM/Propidium Iodide staining

Colorectal cancer cells were grown in ULA plates for 4 days to form spheroids and exposed to small molecule treatment for 3 days. Compound 2 exhibits higher toxicity than compound 1.

HTP Technologies

State of the Art Technologies Available at HTP

a) High-content Imaging and Analysis Instruments
Our systems for high-content imaging (HCI) and high-content analysis (HCA) provide flexible scalability for your research. They feature options and modules to address your specific research including objectives, filters, imaging modes, and environmental conditions. All our systems support a wide range of applications, increased throughput, and we have streamlined workflows.

Operetta CLS High Content Imaging System	Opera Phenix High Content Imaging System	Vectra Polaris Automated Quantitative Pathology System
Powerful LED Light Source Confocal Spinning Disk sCMOS Low Noise Camera 3D Imaging Water Objectives for thick samples Live Cell Chamber PreciScan for Tracking Objects	Solid State Lasers Spinning Disk Confocal 2 Cameras for Speed & Accuracy Large Field of View – 290% Reduce Crosstalk Water Objectives Ideal for 3D Objects Phenologic Application	Multispectral Imaging Automated Slide Scanning FFPE Tissue Sections and TMAs 7–Colour Opal Polaris Compatible Liquid Crystal Tunable Filter (LCTF) MOTiF Technology at 40x magnification 10x – 40x in Brightfield and Fluorescence
ImageStreamX mkII Imaging Flow Cytometer	Li-Cor Odyssey SA Infrared Signal Scanner	High Content Image Analysis
Up to 5000 cell/sec Flexible Sample Prep Easy Interface Recover Unused Sample Analysis Modules for Many Research Areas 96-well Auto-sampler Ideal for non-adherent cells	Highly Sensitive Signal Multi-target at Coordinates Stable Signal Unaffected by Time In-Cell Western System Assay Detection Using IR-Dye	Harmony – HCS building blocks for real-time image feature testing Columbus Image analysis – web-based analysis for batch analysis 50 TB dedicated image server

b) High-throughput Liquid Handling and Assay Instruments
At HTP, we have modular assay automation systems that provide greater flexibility in complex cell-based assay development and screening. We have state-of-the-art instrumentation, for distribution of compound or siRNA/cDNA libraries, and large-scale data analytics solutions.

Agilent – Bravo Benchcel 96- & 384-well formats	PerkinElmer – Janus 96- & 384-well formats	LabCyte – Echo 550 96-, 384- & 1536-well formats	Tecan SPARK Multimode reader
Assay-ready Plates Plate reformatting Barcode Labelling Automated Microplate Sealing HEPA enclosure	HEPA Enclosure Library Reformatting Cherry Picking Cell-based Assay Automation	Fast & Versatile Acoustic Contactless Liq. Transfer Dug Combination Matrix Cherry picking Easy IC50 Setups	Luminescence Fluorescence Monochromator Fluorescence Variable Bandwidth Fluorescence Polarisation Absorbance Alpha Technology

Library Resources

Libraries Available in HTP (Small Molecules & Genome Wide siRNA/shRNA/cDNA)

• Smart collection of small molecule libraries in which targets are annotated significantly, that can be used for target validation/re-purpose studies.
• Small molecule diversity sets.
• Access to SMILE structures for custom sets based on your target(s) or assay needs.
• Genome wide siRNA/shRNA and cDNA libraries for genetic manipulation studies.
• Web-based data analysis portal for pipeline analysis support for your screens-plate uniformity, %CV between your replicates and show you the top hits in waterfall graph.

Milestone Projects

Services

People @ HTP

Dr Giridhanran Periyasamy

Head-HTP

Expert in Automation and Imaging
Expert in Target and Lead Discovery

Shivaji Rikka

Research Manager

Expert in Screening Technology
Expert in Optimising Assay Biology

Matan Thangavelu T

Senior Manager

Expert in HTS and HCS Automation and Imaging
Expert in High Content Image Analysis

Yi Li Gian

Research Associate

Expert in Assay Technology and Miniaturisation
Expert in HT-Liquid Handling